Part:BBa_K4138004:Design
SOPP3-2ABH
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 187
Illegal PstI site found at 316
Illegal PstI site found at 346 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 187
Illegal PstI site found at 316
Illegal PstI site found at 346 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 187
Illegal PstI site found at 316
Illegal PstI site found at 346 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 187
Illegal PstI site found at 316
Illegal PstI site found at 346
Illegal AgeI site found at 1098 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion construct of SOPP3 and 2ABH was designed by our team by linking the two proteins via a spacer sequence.
Source
The individual sequences of the construct components can be found in the following parts:
SOPP3: BBa_K4138001
2ABH: BBa_K4138000
Spacer: BBa_K4138007
References
Torra, J., Lafaye, C., Signor, L. et al. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein. Sci Rep 9, 2428 (2019). https://doi.org/10.1038/s41598-019-38955-3
Christie JM, Salomon M, Nozue K, Wada M, Briggs WR. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. Proc Natl Acad Sci U S A. (1999). https://doi.org/10.1073/pnas.96.15.8779
Z. Wang, A. Choudhary, P.S. Ledvina, F.A. Quiocho, Fine tuning the specificity of the periplasmic phosphate transport receptor. Site-directed mutagenesis, ligand binding, and crystallographic studies., Journal of Biological Chemistry, Volume 269, Issue 40, 1994, Pages 25091-25094, https://doi.org/10.1016/S0021-9258(17)31503-X.