Composite

Part:BBa_K4138004:Design

Designed by: Carlos Classen   Group: iGEM22_Aachen   (2022-09-28)


SOPP3-2ABH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 187
    Illegal PstI site found at 316
    Illegal PstI site found at 346
    Illegal AgeI site found at 1098
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion construct of SOPP3 and 2ABH was designed by our team by linking the two proteins via a spacer sequence.

Source

The individual sequences of the construct components can be found in the following parts:

SOPP3: BBa_K4138001

2ABH: BBa_K4138000

Spacer: BBa_K4138007

References

Torra, J., Lafaye, C., Signor, L. et al. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein. Sci Rep 9, 2428 (2019). https://doi.org/10.1038/s41598-019-38955-3

Christie JM, Salomon M, Nozue K, Wada M, Briggs WR. LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. Proc Natl Acad Sci U S A. (1999). https://doi.org/10.1073/pnas.96.15.8779

Z. Wang, A. Choudhary, P.S. Ledvina, F.A. Quiocho, Fine tuning the specificity of the periplasmic phosphate transport receptor. Site-directed mutagenesis, ligand binding, and crystallographic studies., Journal of Biological Chemistry, Volume 269, Issue 40, 1994, Pages 25091-25094, https://doi.org/10.1016/S0021-9258(17)31503-X.